Publications

* - equal contribution
^# - corresponding author

Extracellular carriers control lipid-dependent secretion, delivery, and activity of WNT morphogens. 
de Almeida Magalhães T^*,  Liu J^*, Chan C, Borges KS, Zhang J, Kane AJ, Weirbowski BM, Ge Y, Liu Z, Mannam P, Zeve D, Weiss R, Breault DT, Huang P, Salic A^#.

DevCell. 2024 Jan 22;59(2):244-261

Screen for Modulation of Nucleocapsid Protein Condensation Identifies Small Molecules with Anti-Coronavirus Activity
Quek RT, Hardy KS, Walker SG, Nguyen DT, de Almeida Magalhães T, Salic A, Gopalakrishnan SM, Silver PA, Mitchison TJ.

ACS Chem Biol. 2023 Mar 17;18(3):583-594

Activation of Hedgehog signaling by the oncogenic RELA fusion reveals a primary cilia-dependent vulnerability in supratentorial ependymoma

de Almeida Magalhães T*, Alencastro Veiga Cruzeiro G, Ribeiro de Sousa G, Englinger B, Fernando Peinado Nagano L, Ancliffe M, Rodrigues da Silva K, Jiang L, Gojo J, Cherry Liu Y, Carline B, Kuchibhotla M, Pinto Saggioro F, Kazue Nagahashi Marie S, Mieko Oba-Shinjo S, Andres Yunes J, Gomes de Paula Queiroz R, Alberto Scrideli C, Endersby R, Filbin MG, Silva Borges K, Salic A, Gonzaga Tone L, Valera ET^#.

Neuro Oncol. 2023 Jan 5;25(1):185-198

Structural basis for catalyzed assembly of the Sonic hedgehog-Patched1 signaling complex.
Huang P^*, Wierbowski BM^*, Lian T^*, Chan C, García-Linares S, Jiang J^#, Salic A^#.
Dev Cell. 2022 Mar 14;57(5):670-685.e8.

Hedgehog pathway modulation by glypican 3-conjugated heparan sulfate.
Liu YC^#, Wierbowski BM, Salic A^#.
J Cell Sci. 2022 Mar 15;135(6):jcs259297.

Structural insights into proteolytic activation of the human Dispatched1 transporter for Hedgehog morphogen release.
Li W*, Wang L*, Wierbowski BM*, Lu M, Dong F, Liu W, Li S, Wang P, Salic A^#, Gong X^#.
Nat Commun. 2021 Nov 29;12(1):6966.

Mechanism and ultrasensitivity in Hedgehog signaling revealed by Patched1 disease mutations
Petrov K, de Almeida Magalhaes T, Salic A^#
Proc Natl Acad Sci U S A. 2021 Feb 9;118(6):e2006800118.

Hedgehog pathway activation requires coreceptor-catalyzed, lipid-dependent relay of the Sonic hedgehog ligand
Wierbowski BM, Petrov K, Aravena L, Gu G, Xu Y, Salic A^#
Dev. Cell. 2020 Oct 6:S1534-5807(20)30719-X. [Epub ahead of print]

Distinct cation gradients power cholesterol transport at different key points in the Hedgehog signaling pathway
Petrov K*, Wierbowski BM*, Liu J, Salic A^#
Dev Cell. 2020 Aug 25:S1534-5807(20)30600-6. [Epub ahead of print]

Structural Basis of Smoothened Activation in Hedgehog Signaling
Huang P*, Zheng S*, Wierbowski BM, Kim Y, Nedelcu D, Aravena L, Liu J, Kruse AC, Salic A^#
Cell. 2018 Jul 12;174(2):312-324.e16. Erratum in: Cell. 2018 Sep 20;175(1):295-297.

Sending and Receiving Hedgehog Signals
Petrov K^#, Wierbowski BM^#, Salic A 
Annu Rev Cell Dev Biol. 2017 Oct 6;33:145-168.
e-print

Mechanism of inhibition of the tumor suppressor Patched by Sonic Hedgehog
Tukachinsky H, Petrov K, Watanabe M, Salic A^#
Proc Natl Acad Sci U S A. 2016 Oct 4;113(40):E5866-E5875.

Cellular cholesterol directly activates Smoothened in Hedgehog signaling
Huang P, Nedelcu D, Watanabe M, Jao C, Kim Y, Liu J, Salic A^#
Cell. 2016 Aug 25;166(5):1176-1187.e14.

DyNAvectors: dynamic constitutional vectors for adaptive DNA transfection
Clima L, Peptanariu D, Pinteala M, Salic A, Barboiu M^#
Chem Commun (Camb). 2015 Dec 25;51(99):17529-31.

Dynamic constitutional frameworks (DCFs) as nanovectors for cellular delivery of DNA
Turin-Moleavin IA, Doroftei F, Coroaba A, Peptanariu D, Pinteala M, Salic A, Barboiu M^#
Org Biomol Chem. 2015 Sep 14;13(34):9005-11.

Bioorthogonal probes for imaging sterols in cells
Jao CY, Nedelcu D, Lopez LV, Samarakoon TN, Welti R, Salic A^#
Chembiochem. 2015 Mar 2;16(4):611-7. 

Biosynthetic labeling and two-color imaging of phospholipids in cells
Jao CY, Roth M, Welti R, Salic A^#
Chembiochem. 2015 Feb 9;16(3):472-6.

Asymmetrical distribution of choline phospholipids revealed by click chemistry and freeze-fracture electron microscopy
Iyoshi S, Cheng J, Tatematsu T, Takatori S, Taki M, Yamamoto Y, Salic A, Fujimoto T^#
ACS Chem Biol. 2014 Oct 17;9(10):2217-22.

Haematopoietic stem cells require a highly regulated protein synthesis rate
Signer RAJ, Magee JA, Salic A, Morrison SJ^#
Nature. 2014 May 1;509(7498):49-54.

Oxysterol binding to the extracellular domain of Smoothened in Hedgehog signaling
Nedelcu D*, Liu J*, Xu Y, Jao C, Salic A^#
Nat Chem Biol. 2013 Sep;9(9):557-64.

Dispatched and scube mediate the efficient secretion of the cholesterol-modified hedgehog ligand
Tukachinsky H, Kuzmickas RP, Jao CY, Liu J, Salic A^#
Cell Rep. 2012 Aug 30; 2(2):308-20.

Imaging protein synthesis in cells and tissues with an alkyne analog of puromycin
Liu J, Xu Y, Stoleru D, Salic A^#
Proc Natl Acad Sci U S A. 2012 Jan 10;109(2):413-8.

Processing and turnover of the Hedgehog protein in the endoplasmic reticulum
Chen X*, Tukachinsky H*, Huang CH, Jao C, Chu YR, Tang HY, Mueller B, Schulman S, Rapoport TA, Salic A^#
J Cell Biol. 2011 Mar 7;192(5):825-38.

A mechanism for vertebrate Hedgehog signaling: recruitment to cilia and dissociation of SuFu-Gli protein complexes
Tukachinsky H*, Lopez LV*, Salic A^#
J Cell Biol. 2010 Oct 18;191(2):415-28.

Metabolic labeling and direct imaging of choline phospholipids in vivo
Jao CY, Roth M, Welti R, Salic A^#
Proc Natl Acad Sci U S A. 2009 Sep 8;106(36):15332-7.

Exploring RNA transcription and turnover in vivo by using click chemistry
Jao CY, Salic A^#
Proc Natl Acad Sci U S A. 2008 Oct 14;105(41):15779-84.

Detection of S-phase cell cycle progression using 5-ethynyl-2'-deoxyuridine incorporation with click chemistry, an alternative to using 5-bromo-2'-deoxyuridine antibodies
Buck SB, Bradford J, Gee KR, Agnew BJ^#, Clarke ST, Salic A
Biotechniques. 2008 Jun;44(7):927-9.

A chemical method for fast and sensitive detection of DNA synthesis in vivo
Salic A^#, Mitchison TJ
Proc Natl Acad Sci U S A. 2008 Feb 19;105(7):2415-20.

Earlier Work

Identifying small molecule inhibitors of the ubiquitin-proteasome pathway in Xenopus egg extracts
Salic A*, King RW*
Methods Enzymol. 2005;399:567-85.

Vertebrate shugoshin links sister centromere cohesion and kinetochore microtubule stability in mitosis
Salic A^#, Waters JC, Mitchison TJ
Cell. 2004 Sep 3;118(5):567-78.

The chromosomal passenger complex is required for chromatin-induced microtubule stabilization and spindle assembly
Sampath SC, Ohi R, Leismann O, Salic A, Pozniakovski A, Funabiki H^#
Cell. 2004 Jul 23;118(2):187-202.

The roles of APC and Axin derived from experimental and theoretical analysis of the Wnt pathway
Lee E*, Salic A*, Krüger R, Heinrich R^#, Kirschner MW^#
PLoS Biol. 2003 Oct;1(1):E10. Erratum in: PLoS Biol. 2004 Mar;2(3):E89.

Anteroposterior patterning in hemichordates and the origins of the chordate nervous system
Lowe CJ^#, Wu M, Salic A, Evans L, Lander E, Stange-Thomann N, Gruber CE, Gerhart J^#, Kirschner M
Cell. 2003 Jun 27;113(7):853-65.

A novel centrosome-associated protein with affinity for microtubules
Stein PA, Toret CP, Salic AN, Rolls MM, Rapoport TA^#
J Cell Sci. 2002 Sep 1;115(Pt 17):3389-402.

A novel set of Wnt-Frizzled fusion proteins identifies receptor components that activate beta-catenin-dependent signaling
Holmen SL*, Salic A*, Zylstra CR, Kirschner MW, Williams BO^#
J Biol Chem. 2002 Sep 20;277(38):34727-35.

Physiological regulation of [beta]-catenin stability by Tcf3 and CK1epsilon
Lee E*, Salic A*, Kirschner MW^#
J Cell Biol. 2001 Sep 3;154(5):983-93.

Inhibition of Cdh1-APC by the MAD2-related protein MAD2L2: a novel mechanism for regulating Cdh1
Pfleger CM*, Salic A*, Lee E*, Kirschner MW^#
Genes Dev. 2001 Jul 15;15(14):1759-64.

HIFalpha targeted for VHL-mediated destruction by proline hydroxylation: implications for O2 sensing
Ivan M, Kondo K, Yang H, Kim W, Valiando J, Ohh M, Salic A, Asara JM, Lane WS, Kaelin WG Jr.^#
Science. 2001 Apr 20;292(5516):464-8.

Control of beta-catenin stability: reconstitution of the cytoplasmic steps of the wnt pathway in Xenopus egg extracts
Salic A*, Lee E*, Mayer L, Kirschner MW^#
Mol Cell. 2000 Mar;5(3):523-32.

Geminin, a neuralizing molecule that demarcates the future neural plate at the onset of gastrulation
Kroll KL^#, Salic AN, Evans LM, Kirschner MW
Development. 1998 Aug;125(16):3247-58.

Sizzled: a secreted Xwnt8 antagonist expressed in the ventral marginal zone of Xenopus embryos
Salic AN, Kroll KL, Evans LM, Kirschner MW^#
Development. 1997 Dec;124(23):4739-48.